Nucleic acid detection using BRET-beacons based on bioluminescent protein-DNA hybrids

Article

Engelen, W., van de Wiel, K.M., Meijer, L.H.H., Saha, B. & Merkx, M. (2017). Nucleic acid detection using BRET-beacons based on bioluminescent protein-DNA hybrids. Chemical Communications, 53(9), 2862-2865. In Scopus Cited 1 times.

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Abstract

 


Bioluminescent molecular beacons have been developed using a modular design approach that relies on BRET between the bright luciferase NanoLuc and a Cy3 acceptor. While classical molecular beacons are hampered by background fluorescence and scattering, these BRET-beacons allow detection of low pM concentrations of nucleic acids directly in complex media.

Graphical abstract: Nucleic acid detection using BRET-beacons based on bioluminescent protein–DNA hybrids