Beyond the already impressive functional diversity of natural proteins
Our group operates at the interface of chemical biology and synthetic biology. Combining protein engineering and DNA nanotechnology, we develop intelligent biomolecular sensors and switches for applications in intracellular imaging, optogenetics, point-of-care diagnostics and antibody-based therapies. An important research theme is to develop generic engineering concepts for the development of protein-based switches, which include fluorescent sensors for intracellular imaging of metal ions, photo-switchable proteins, and protein-based sensors for antibody detection and actuation. We have an active policy of sharing our protein building blocks by depositing our plasmids to AddGene and promote their use by writing dedicated protocols and tutorial reviews. In education, our main goal is to train bachelor, masters and PhD students to become creative biomolecular engineers and independent critical scientists.
FRET and BRET sensors for intracellular imaging
An important area of activity has been the development of fluorescent sensor proteins based on Förster Resonance Energy Transfer (FRET).
Bioluminescent sensors for point-of-care diagnostics
A second line of research is the development of sensor proteins for point-of-care diagnostics.
Protein sensing and actuation using DNA-based molecular circuits
DNA has emerged as a highly versatile construction material for nanometer-sized structures and sophisticated molecular machines and...
Meet some of our Researchers
Our most recent peer reviewed publications
Too creative for our own goodACS Sensors (2019)
Ratiometric bioluminescent sensor proteins based on intramolecular split luciferase complementationACS Sensors (2019)
Paper-based antibody detection devices using bioluminescent BRET-switching sensor proteinsAngewandte Chemie - International Edition (2018)
Protease-activatable scaffold proteins as versatile molecular hubs in synthetic signaling networksACS Synthetic Biology (2018)
Accelerating DNA-based computing on a supramolecular polymerJournal of the American Chemical Society (2018)