Plasmids, Proteins and Protocols

We have an active policy of sharing our protein building blocks by contributing depositing our plasmids to AddGene and promote their use by writing dedicated protocols and tutorial reviews.

Plasmids available through Addgene

• eCALWY: original CFP/YFP-based FRET sensors for Zn²⁺
• redCALWY: red variants of eCALWY sensors using Orange/Cherry FRET pair
• eZinCh^-2: genictally encoeded FRET sensors for Zn²⁺(cytosolic, ER, mitochondria)
• BL-ZinCh1; dual readout BRET/FRET sensors for measuring intracellular Zn²⁺
• MagFRET: FRET sensor for intracellular Mg²⁺imaging
• CLY1-9:FRET reference standard. Fusion of eCFP and eYFP with flexible linker ranging between 1 and 9 GGSGGS repeats
• CNA35-FP: colorful fluorescent proteins for collagen imaging

Protocols

• FRET sensors for intracellular Zn2+ imaging
  • http://www.nature.com/protocolexchange/protocols/609
  • http://pubs.rsc.org/en/content/articlehtml/2015/mt/c4mt00179f
• BRET sensor engineering
  • R, Arts, S.J.A. Aper, M. Merkx. Engineering BRET sensor proteins Meth. Enzymol. (2017) in press
• Intracellular imaging of bile acid homeostasis and transport
  • https://www.jove.com/video/53659/real-time-monitoring-intracellular-bile-acid-dynamics-using
• Chemoselective protein functionalization of sensor surfaces
  • E.H.M. Lempens, B.A. Helms, M. Merkx Chemoselective protein and peptide immobilization on biosensor surfaces Meth. Mol. Biol. (2011) 751, 401-420.
• Protein-DNA conjugation
  • W. Engelen, M. Merkx DNA-specific biosensors based on intramolecular β-lactamase-inhibitor complex formation Meth. Mol. Biol. (2017) in press

Other plasmids

If you are interested in plasmids currently not distributed via Addgene please send your request to Prof. Dr Maarten Merkx.