Laboratory for Cell & Tissue Engineering

The Laboratory for Cell & Tissue Engineering is located at Gemini-South 4.02 and includes the Microscopy Laboratory.

Founded in 2001, this laboratory now has grown to a state-of-the-art laboratory enabling high-end research in the field of tissue engineering at the full spectrum of the topic. Currently housing over 120 researchers, this service laboratory has become the key feature of cellular research at the department of Biomedical Engineering.

Its main features comprise of a fully equipped cell culture room, containing multiple safety cabinets and cell incubators. This part of the laboratory offers ample space for elaborate experimental tissue engineering. No less than 6 separate safety cabinets and over 15 CO2 incubators harbor multiple independent researchers of the different research groups within the department of Biomedical Engineering. A variety of bioreactors and mechanical or electrical stimulation equipment allow research on every aspect of engineering, load-bearing tissue with specific mechanical characteristics.

The remaining laboratory is filled with a fully equipped biochemical room. A vast amount of standard chemical and biochemical substances allow for a wide range of assays including read-out (e.g. FACS, qPCR, luminescence, fluorescence and absorbance reader, etc). For more tissue based assays, a cryotome, microtomes and a vibratome are added to the equation, together with a variety of histological and (immune)histochemical possibilities. Furthermore, this part of the laboratory contains possibilities to prepare scaffolding materials using electrospinning and mechanically challenge tissues and materials using uniaxial or biaxial tensile testers. Hydrodynamic pulsatile and durability testers further support qualitative testing of tissue-engineered heart valves.

A fully blindable room completes the analytical facilities. Several fluorescence, confocal and 2-photon microscopes enable the visualization of cell and tissue organization. Combined with mechanical equipment (rheometer, indentor) and/or life cell facilities (incubator), tissues can be microscopically observed during stress or strain. 

Key features:

  • BDC Labs Hydrodynamic pulsatile and durability tester for (tissue-engineered) heart valves
  • Supercontinuum Confocal Leica TCS SP5 X equipped with UV, WLL and IR
  • Keyence digital microscope with automated z-stage and 100-1000x zoom lens
  • IME Electrospin boxes, temperature and humidity controlled
  • Full culture facilities, with rollerbottle systems for the culture of vast amounts of cells and with FlexWell set-ups for mechanical stimulation
  • Tensile testers, Bose Uniaxial, Cellscale Biaxial
  • FACS, qPCR, UV/ECL imager, platereader (abs,fluoresc, luminesc)
  • Certified for working with human or animal tissues and genetically modified organisms according to the ML-II classification in Dutch law