Ir. Bas Rosier
Bas Rosier (1990) received his BSc and MSc degrees in Biomedical Engineering at Eindhoven University of Technology. For his Master thesis he investigated the use of microfluidic devices in the fields of supramolecular chemistry and chemical biology and developed a microfluidic gradient generator to determine kinetic parameters of enzymes. During an internship at the Wyss Institute for Biologically Inspired Engineering at Harvard University, he worked in the group of dr. W.M. Shih on the development of a fluorescence barcoding system for a microfluidic DNA origami-scaffolded, SNARE-mediated vesicle fusion assay.
Currently, he is a PhD candidate under supervision of dr. T.F.A. de Greef and prof. L. Brunsveld and uses DNA nanotechnology to study kinetics of multi-enzyme complexes and cascades. In the cell, functionally related enzymes in signaling pathways and metabolic reaction cascades are often spatiotemporally localized to increase throughput, efficiency and specificity. Using rectangular DNA origami scaffolds to control the organization of enzymes with nanometer-precision, the effects on reaction kinetics and cascade efficiency can be investigated in a modular fashion.