Publications

2017

114. L.H.H. Meijer, A. Joesaar, E. Steur, W. Engelen, R. A. van Santen, M. Merkx. T.F.A. de Greef
Hierarchical control of enzymatic actuators using DNA-based switchable memories
Nature Communications (2017), 8, in press

113. B.J.H.M. Rosier, G.A.O. Cremers, W. Engelen, M. Merkx, L. Brunsveld, T.F.A. de Greef
Incorporation of native antibodies and Fc-fusion proteins on DNA nanostructures via a modular conjugation strategy
Chem. Commun. (2017) 53, 7393-7396

112. A. den Hamer, P. Dierickx, R. Arts, J.S.P.M. de Vries, L. Brunsveld, M. Merkx
Bright bioluminescent BRET sensor proteins for measuring intracellular caspase activity
ACS Sensors (2017) 2, 729-734

111. M. Merkx
SensUs: Challenging the next generation of sensor scientists
ACS Sensors (2017) 2, 613-613  (editorial)

110. M. Merkx, A.J. Pierik
Editorial overview: Nine short stories of metals in biology
Curr. Opin. Chem. Biol. (2017) 37, vi-vii  (editorial)

109. R. Arts, S.J.A. Aper, M. Merkx
Engineering BRET sensor proteins
Meth. Enzymol. (2017) 589, 87-114

108. W. Engelen, K.M. Wiel, L.H.H. Meijer, B. Saha, M. Merkx
Nucleic acid detection using BRET-beacons based on bioluminescent protein-DNA hybrids
Chem. Commun. (2017) 53, 2862-2865

107. W. Engelen, L.H.H. Meijer, B. Somers, T.F.A. de Greef, M. Merkx
Antibody-controlled actuation of DNA-based molecular circuits
Nature Communications (2017) 8, 14473

106. M. van Rosmalen, B.M.G. Janssen, N.M. Hendrikse, A.J. van der Linden, P.A. Pieters, D. Wanders, T.F.A. de Greef, M. Merkx
Affinity maturation of a cyclic peptide handle for therapeutic antibodies using deep mutational scanning
J. Biol. Chem. (2017) 292, 1477-1489

105. W. Engelen, M. Merkx
DNA-specific biosensors based on intramolecular β-lactamase-inhibitor complex formation
Meth. Mol. Biol. (2017) 1596, 179-194

104. A. den Hamer, L.J.M. Lemmens, M.A.D. Nijenhuis, C. Ottmann,, M. Merkx, T.F.A. de Greef, L. Brunsveld
Small Molecule-Induced and Cooperative Enzyme Assembly on a 14-3-3 Scaffold
ChemBioChem (2017)  18, 331-335

2016

103. M. Merkx
Opportunities for protein engineering in sensing
ACS Sensors (2016) 1, 1280-1280                (editorial)

102. S. Baldivia, A. Levy, S. Hedge, S.J.A. Aper, M. Merkx, R. Grytz
A novel organ culture model to quantify collagen remodeling in tree shrew sclera
PLOS One (2016) 11, e0166644

101. S.J.A. Aper, P. Dierickx, M. Merkx
Dual readout BRET/FRET-sensors for measuring intracellular zinc
ACS Chem. Biol. (2016) 11, 2854-2864

100. R.P.G. Bosmans, J.M. Briels, L.-G. Milroy, T.F.A. de Greef, M. Merkx, L. Brunsveld
Supramolecular control over split-luciferase complementation
Angew. Chem. Int. Ed. (2016) 55, 8899-8903

99. A.M. Hessels, M. Merkx
Simple method for proper analysis of FRET sensor titration data and intracellular imaging experiments based on isosbestic points
ACS Sensors (2016) 1, 498-502

98. R. Arts, I. den Hartog, S. Zijlema, V. Thijssen, S. v.d. Beelen, M. Merkx
Detection of antibodies in blood plasma using bioluminescent sensor proteins and a smartphone
Anal. Chem. (2016) 88, 4525-4531

97. S.J.A. Aper, M. Merkx
Rewiring multi-domain protein switches: transforming a fluorescent Zn2+-sensor into a light-responsive Zn2+ binding protein
ACS Synth. Biol. (2016) 5, 698-709

96. W. Engelen, B.M.G. Janssen, M. Merkx
DNA-based control of protein activity
Chem. Commun. (2016) 52, 3598-3610            (featured on front cover)

95. M. Moirangthem, R. Arts, M. Merkx, A.P.H.J. Schenning
An optical sensor based on a photonic polymer film to detect calcium in serum
Adv. Funct. Mat. (2016) 26, 1154-1160             (featured on front cover)

94. A.M. Hessels, K.M. Taylor, M. Merkx
Monitoring cytosolic and ER Zn2+ in stimulated breast cancer cells using genetically encoded FRET sensors
Metallomics (2016) 8, 211-217

93. S.M.W. van de Wiel. M. Merkx, S.F.J. van de Graaf
Real time monitoring of intracellular bile acid dynamics using a genetically encoded FRET-based Bile Acid Sensor
JoVE (2016) 107, e53659

2015

92. J. Wang, C. Luo, C. Shan, Q. You, J. Lu, S. Elf, Y. Zhou, Y. Wen, J.L. Vinkenborg, J. Fan, H. Kang,
R. Lin, D. Han, Y. Xie, J. Karpus, S. Chen, S., Ouyang, C. Luan, N. Zhang, H. Ding, M. Merkx, H. Liu,
J. Chen, H. Jiang, C. He
Inhibition of human copper trafficking by small molecule significantly attenuates cancer cell proliferation
Nature Chemistry (2015) 7, 968-979

91. A.M. Hessels, P. Chabosseau, M.H. Bakker, W. Engelen, G.A. Rutter, K.M. Taylor, M. Merkx
eZinCh-2: a versatile, genetically-encoded FRET sensor for cytosolic and intra-organelle Zn2+ imaging
ACS Chemical Biology (2015) 10, 2126-2134

90. B.M.G. Janssen, S.P.F.L. van Ommeren, M. Merkx
Efficient synthesis of peptide and protein functionalized pyrrole-imidazole polyamides using native chemical ligation
Int. J. Mol. Sci. (2015) 16, 12631-12647

89. B.M.G. Janssen, M. van Rosmalen, L. van Beek, M. Merkx
Antibody activation using DNA-based logic gates
Angew. Chemie Int. Ed. (2015) 54, 25302533                  (hot paper)

88. A.M. Hessels, M. Merkx
Genetically-encoded FRET-based sensors for monitoring Zn2+ in living cells
Metallomics (2015) 7, 258-266                         (cover)

87. B.M.G. Janssen, W. Engelen, M. Merkx
DNA-directed control of enzyme-inhibitor complex formation: a modular approach to reversibly switch enzyme activity
ACS Synthetic Biology (2015) 4, 547-553

2014

86. S.J.A. Aper, A. C.C. van Spreeuwel, M.C. van Turnhout, A.J. van der Linden, P.A. Pieters, N.L.L. van der Zon, S.L. de la Rambelje, C.V.C. Bouten, M. Merkx
Colorful protein-based fluorescent probes for collagen imaging
PLOS One (2014)  9, e114983

85. L. H. Lindenburg, M. Malisauskas, L. van Oppen, T. Sips, S.F.J. van de Graaf, M. Merkx
Quantifying stickiness; thermodynamic characterization of intramolecular domain interactions to guide the design of FRET sensors
Biochemistry (2014) 53, 6370-6381

84. M. Merkx
Making sense of biosensors
ChemBioChem (2014) 15, 2467-2468

83. P. Chabosseau, E. Tuncay, G. Meur, E.A. Bellomo, A.M. Hessels, S. Hughes, P.R.V. Johnson, M. Bugliani, P. Marchetti, A.R. Lyon, M. Merkx, G.A. Rutter
Mitochondrial and ER-targeted eCALWY probes reveal high levels of free Zn2+
ACS Chemical Biology (2014) 9, 2111-2120

82. L.H. Lindenburg, M. Merkx
Engineering genetically encoded FRET sensors
Sensors (2014) 14, 11691-11713

81. A.M. Hessels, M.Merkx
Geneticall-encoded zinc sensors for intracellular imaging and diagnostics
Book chapter in Zinc Signals in Cellular Functions and Disorders, in press (Springer, 2014)

80. M. Merkx
Modular engineering of protein switches
Book chapter in Synthetic Biology, volume 1, 164-186 (Royal Society of Chemistry, 2014)

79. V. Lanquar, G, Grossmann, J.L. Vinkenborg, M. Merkx, S. Thomine, W.B. Frommer
Dynamic imaging of cytosolic zinc in Arabidopsis roots combining FRET sensors and RootChip technology
New Phytologist  (2014)  202, 198-208

2013

78. L.H. Lindenburg, J.L. Vinkenborg1, J. Oortwijn, S.J.A. Aper, M. Merkx
MagFRET: the first genetically encoded fluorescent Mg2+ sensor
PLOS One (2013) 8, e82009

77. S. Banala, R. Arts, S.J.A. Aper, M. Merkx
No washing, less waiting: engineering biomolecular reporters for single-step antibody detection in solution
Org. Biomol. Chem. (2013) 11, 2127-2132                (featured on the front cover)

76. M. Merkx, M.V. Golynskiy, L.H. Lindenburg, J.L. Vinkenborg
Rational design of FRET sensor proteins based on mutually exclusive domain interactions
Biochemical Soc. Trans. (2013) 41, 1201–1205

75.  L.H. Lindenburg, A.M. Hessels, E.H.T.M. Ebberink, R. Arts, M. Merkx
Robust red FRET sensors using self-associating fluorescent domains
ACS Chemical Biology (2013) 8, 2133-2139

74. S. Banala, S.J.A. Aper, W. Schalk, M. Merkx
Switchable reporter enzymes based on mutually exclusive domain interactions allow antibody detection directly in solution
ACS Chemical  Biology (2013) 8, 2127-2132

73. B.M.G. Janssen, E.H.M. Lempens, L.L.C. Olijve, I.K. Voets, J.L.J. van Dongen, T.F.A. de Greef, M. Merkx
Reversible blocking of antibodies using bivalent peptide-DNA conjugates allows protease-activatable targeting
Chemical Science (2013) 4, 1442-1450                 (featured on the back-cover)

72. D.T. Dang, H.D. Nguyen, M. Merkx, L. Brunsveld
Supramolecular control of enzyme activity through cucurbit[8]uril mediated dimerization
Angewandte Chemie (2013) 125, 2987 –2991

71. M.S. Koay, B.M.G. Janssen, M. Merkx.
Tuning the metal binding site specificity of a fluorescent sensor protein: from copper to zinc and back
Dalton Transactions (2013) 42, 3230 – 3232             (featured on the inside front cover)

70. L.M. van der Velden, M.V. Golynskiy, S.W.C. van Mil, L.W.J. Klomp, M. Merkx. S.F.J. van de Graaf
Genetically encoded FRET sensors to monitor bile acid dynamics in living cells
Hepatology (2013) 75, 740-752                     (featured on the front cover)

2012

69. T. Ansbacher, H. K. Srivastava, M. Merkx, A. Shurki
Calculation of transition dipole moment in fluorescent proteins–towards efficient energy transfer
Phys. Chem. Chem. Phys. (2012) 14, 4109–4117

68. L. Lindenburg, M. Merkx
Colorful calcium sensors
ChemBioChem (2012) 13, 349-351

2011

67. A. Klink, J. Heynens, B. Herranz, H.M.H.F. Sanders, G.J. Strijkers, M. Merkx, K. Nicolay, V. Fuster, Z. Mallat, W.J.M. Mulder, Z.A. Fayad
In vivo characterization of a new abdominal aortic aneurysm mouse model with conventional and molecular MRI
J. Am. Col. Cardiol. (2011) 58, 2522-2530

66. J.L. Vinkenborg, S.M.J. van Duijnhoven, M. Merkx
Reengineering of a chelating fluorescent zinc sensor yields the first genetically encoded Cd2+ probe
Chem. Comm., 2011, 47, 11879–11881

65.  M. Breurken, E.H.M. Lempens, E.W. Meijer, M. Merkx
Semi-synthesis of a protease-activatable collagen targeting probe
Chem. Comm. (2011) 47, 7998-8000

64. M. S. Shoshan, D. E. Shalev, W. Adriaens, M. Merkx, T. M. Hackeng, E. Y. Tshuva
NMR Characterization of a Cu(I)-bound Peptide Model of Copper Metallochaperones: Insights on the Role of Methionine
Chem. Comm. (2011) 47, 6407-6409

63. V.M. Hernández-Rocamora, S.W.A Reulen, B. de Waal, E.W. Meijer, J.M. Sanz, M. Merkx
Choline dendrimers as generic scaffolds for the non-covalent synthesis of multivalent protein assemblies
Chem. Comm.  (2011) 47, 5997-5999    

62. M.M.C. Bastings, B.A. Helms, I. van Baal, T.M. Hackeng, M. Merkx, E. W. Meijer
From phage display to dendrimer display: insights into multivalent binding
J. Am. Chem. Soc. (2011) 133, 6636-6641

61. M. Golynskiy, M.S. Koay, J.L. Vinkenborg, M. Merkx
Engineering protein switches: sensors, regulators, and spare parts for biology
and biotechnology
ChemBioChem (2011) 12, 353-361

60. E.H.M. Lempens, L. Agemy, M. Merkx, M. Tirrell, E.W. Meijer
Dendrimer display of tumor-homing peptides
Bioconjugate Chem. (2011) 22, 397-405

59. M. Breurken, E.H.M. Lempens, R.P. Temming, B.A. Helms, E.W. Meijer, M. Merkx
Collagen targeting using multivalent protein-functionalized dendrimers
Biorg. Med. Chem. (2011) 19, 1062-1071

58. E.H.M. Lempens, B.A. Helms, M. Merkx
Chemoselective protein and peptide immobilization on biosensor surfaces
Meth. Mol. Biol. (2011) 751, 401-420.

57. H.M.H.F. Sanders, M.Iafisco, E.M. Pouget, P.H.H. Bomans, F.Nudelman, G. Falini, G. de Width, M. Merkx, G.J. Strijkers, K. Nicolay, N.A.J.M. Sommerdijk
The binding of CNA35-based contrast agents to collagen fibrils
Chem. Comm. (2011) 47, 1503-1505

2010

56. M. Golynskiy, W.F. Rurup, M. Merkx
Antibody detection using a FRET-based protein conformational switch
ChemBioChem (2010) 11, 2264-2267

55. M. Breurken, E.H.M. Lempens, M. Merkx
Protease-activatable collagen targeting based on protein cyclization
ChemBioChem (2010) 11, 1665-1668

54. M. M. C. Bastings, T.F.A. de Greef, M. Merkx and E. W. Meijer
The supramolecular polymerization of flexible AB-type biological building blocks: A quantitative analysis
Chemical Science 1, (2010) 1, 79-88

53. J.L. Vinkenborg, M.S. Koay, M. Merkx
Fluorescent imaging of transition metal homeostasis using genetically-encoded sensors
Curr.Opin.Chem.Biol. (2010) 14, 232-237

52. S.W.A. Reulen, M. Merkx
Exchange kinetics of protein-functionalized micelles and liposomes studied by Förster Resonance Energy Transfer
Bioconjugate Chemistry .(2010) 21, 860-866

51. E.H.M.Lempens, B.A. Helms, A. Bayles, M. Merkx, E.W. Meijer (2010)
A versatile, modular platform for multivalent peptide ligands based on a dendritic wedge
Eur. J. Org. Chem.(2010)  2010, 111-119

2009

50. Helms, B., Reulen, S.W.A., Nijhuis, S., de Graaf-Heuvelmans, P.T.H.M., Merkx, M., Meijer, E.W.
High-affinity peptide-based collagen targeting using synthetic phage mimics: from phage display to dendrimer display
J. Am. Chem, Soc. (2009) 131, 11683-11685

49. S.W.A. Reulen, I. van Baal, J.M.H. Raats, M. Merkx,
Efficient, chemoselective synthesis of immunomicelles using single-domain antibodies with a C-terminal thioester
BMC Biotechnology. (2009)  9, 66

48. J.L. Vinkenborg, T.J. Nicolson, E.A. Bellomo, M.S. Koay, G.A. Rutter, and M. Merkx
Imaging of intracellular free Zn2+ in real time using genetically-encoded FRET sensors
Nature Methods (2009) 6, 737-740

47. E.H.M. Lempens, I. van Baal, J.L.J. van Dongen, T.M. Hackeng, M. Merkx, E.W. Meijer
Non-covalent synthesis of multivalent protein dendrimers
Chemistry (2009) 15, 8760-8767

46 S.W.A. Reulen, P.W. Bomans, P.Y.W. Dankers, E.W. Meijer, M. Merkx,
Collagen targeting using multivalent protein micelles: the strength of multiple weak interactions
J. Am. Chem. Soc. (2009), 131, 7304-7312

45. M. Merkx
Rational design of FRET-based sensor proteins
Annual Reviews in Fluorescence 2008

44. H.M.H.F. Sanders, G.J. Strijkers, W.J.M. Mulder, H.P. Huinink, S.J.F. Erich, O.C.G. Adan, N.J.A.M. Sommerdijk, M. Merkx and K. Nicolay
Morphology, binding behavior and MR-properties of paramagnetic collagen binding liposomes
Contrast Media and Molecular Imaging. (2009) 4, 81-88

43. E.H.M. Lempens, B.A. Helms, M. Merkx, and E.W. Meijer
Efficient and chemoselective immobilization of proteins by using aniline-catalyzed oxime chemistry
ChemBioChem, (2009) 410, 658-662

42. Hernández-Rocamora, V.M., Maestro, B., de Waal, B., Morales, M., García, P., Meijer, E.W., Merkx, M., Sanz, J.M.
Multivalent choline dendrimers as potent inhibitors of Pneumococcal cell wall hydrolysis
Angewandte Chemie Int. Ed, (2009) 48, 948-951        (VIP paper)

2008

41. M.M.C. Bastings, I. van Baal, E.W. Meijer and M. Merkx
One-step refolding and purification of disulfide-containing proteins with a C-terminal MESNA thioester
BMC Biotechnology (2008) 8, 76

40. R.T.A. Megens,  M.G.A. Oude Egbrink, M. Merkx, D.W. Slaaf, M.A.M.J. van Zandvoort
Two-photon microscopy on vital carotid arteries: imaging the relation between collagen and inflammatory cells in atherosclerotic plaques
 J. Biomed. Optics (2008) 13, 044022
 
39. T.H. Evers, M.A.M. Appelhof, E.W. Meijer, M. Merkx, 
His-tags as Zn(II) binding motifs in a protein-based fluorescent sensor
Protein Engineering Design and Selection  (2008) 21, 529-536

38. H.P. Huinink, H.M.H.F. Sanders, S.J.F. Erich, K. Nicolay, G.J. Strijkers, M. Merkx and O.C.G Adan High resolution NMR imaging of paramagnetic liposomes targeted to functionalized surface
Magn. Res. Med. (2008) 59, 1282-1286

37. A.M.A. Mingels, J.L.J. van Dongen, M. Merkx
Mapping preferred sites for fluorescent labeling by combining fluorescence and MS analysis of tryptic CNA35 protein digests
J. Chromatography B. (2008) 863, 293-297

2007

36. Evers, T.H., Appelhof, M.A.M., de Graaf-Heuvelmans, P.T.H.M., Meijer, E.W., Merkx, M.
Sensitive ratiometric detection of Zn(II) using chelating fluorescent protein chimeras.
J. Mol. Biol. (2007) 374, 411-425

35. Helms, B.,  van Baal, I., Merkx, M., Meijer, E.W.  
Site-specific protein and peptide immobilization on a biosensor surface via pulsed native chemical ligation ChemBioChem (2007) 8, 1790-1794    

34. S.M. Chafekar, H. Malda, M. Merkx, E.W. Meijer, D. Viertl, H.L. Lashuel, F. Baas, W. Scheper Branched KLVFF tetramers strongly potentiate inhibition of amyloid aggregation
ChemBioChem (2007) 8, 1857-1864    

33. P.E. van der Berghe, D.E. Folmer, H.E.M. Malingre, E. van Beurden, A. Klomp, B. van der Sluis, M. Merkx, R. Berger and L.W.J. Klomp
Human copper transporter 2 is localized in late endosomes and lysosomes and facilitates cellular copper uptake
Biochem. J. (2007) 407, 49-59        

32. T.H. Evers, J.L.J. van Dongen, E.W. Meijer, M. Merkx
Ligand-induced monomerization of cytochrome c' studied using native mass spectrometry and fluorescence resonance energy transfer
J. Biol. Inorg. Chem. (2007) 12, 919-928        

31. R.A. Boerboom, K. Nask-Krahn, R. Megens, M.A.M.J. van Zandvoort, M. Merkx, C.V.C. Bouten
High resolution imaging of collagen architecture and synthesis using a versatile collagen probe
J. Struct. Biol. (2007) 159, 392-399        

30. De Groot, M.T., Merkx, M., Koper, M.T.M.
Evidence for heme release in layer-by-layer assemblies of myoglobin and polystyrenesulfonate on pyrolytic graphite
J. Biol. Inorg. Chem. (2007) 12, 761-766  

29. Megens, R R.T.A., Oude Egbrink, M.G.A., Cleutjens, J., Kuijpers, M.J.E., Schiffers, P.H.M., Merkx, M., Slaaf, D.W., van Zandvoort, M.A.M.J.
Imaging collagen in intact viable healthy and atherosclerotic arteries using fluorescently-labeled CNA35 and two-photon laser scanning microscopy
Molecular Imaging (2007) 6, 247-260

28. J.L. Vinkenborg, T.H. Evers, S.W.A. Reulen, E.W. Meijer, M. Merkx
Enhanced sensitivity of FRET-based protease sensors by redesign of the GFP dimerization interface
ChemBioChem (2007) 8, 1119-1121

27. De Groot, M.T., Merkx, M., Koper, M.T.M.
Reorganization of immobilized horse and yeast cytochrome c induced by pH changes or nitric oxide binding
Langmuir (2007) 23, 3832-3839    

26. van Dongen, E.M.W.M, Evers, T.H., Dekkers, L.M., Meijer, E.W., Klomp, L.W.J., Merkx, M. Variation of linker length in ratiometric fluorescent sensor proteins allows rational tuning of Zn(II) affinity in the picomolar to femtomolar range
J. Am. Chem. Soc. (2007)  129, 3494-3495        

25. De Groot, M.T., Merkx, M., Koper, M.T.M.
Bioinspired electrocatalytic reduction of nitric oxide by immobilized heme groups
Compte Rendu Chimie (2007) 10, 414-420

24. Reulen, S.W.A., Brusselaars, W.W.T., Langereis, S., Mulder, W.J.M., Breurken, M., Merkx, M. Protein-liposome conjugates using cysteine-lipids and native chemical ligation
Bioconjugate Chem. (2007) 18, 590-596    

23. De Groot, M.T., Evers, T.H. Merkx, M., Koper, M.T.M.
Electron transfer and ligand binding to cytochrome c’ immobilized on self-assembled monolayers
Langmuir (2007) 23, 729-736    

2006

22. Evers, T.H., van Dongen, E.M.W.M., Faesen, A.C., Meijer, E.W., Merkx, M.
Quantitative understanding of energy transfer between fluorescent proteins connected via flexible peptide linkers
Biochemistry (2006) 45, 13183-13192

21. van Dongen, E.M.W.M, Dekkers, L.M., Spijker, K., Meijer, E.W., Klomp, L.W.J., Merkx, M. Ratiometric fluorescent sensor proteins with subnanomolar affinity for Zn2+ based on copper chaperone domains
J. Am. Chem. Soc. (2006) 128, 10753-10762

20. de Groot, M.T., Merkx, M., Koper, M.T.M.
Additional evidence for heme release in myoglobin-DDAB films on pyrolytic graphite
Electrochem. Comm. (2006) 8, 999-1004

19. Nash-Krahn, K., Bouten, C.V.C., van Tuijl, S., van Zandvoort, M.A.J.M., Merkx, M.
Fluorescently labeled collagen binding proteins allow specific visualization of collagen in tissues and live cell culture
Anal. Biochem. (2006) 350, 177-185  

2005

18. de Groot, M.T., Merkx, M., Koper, M.T.M.
Heme release in myoglobin-DDAB films and its role in electrochemical NO reduction
J. Am. Chem. Soc. (2005) 127, 16224-16232     
 
17. van Baal, I., Malda, H., Synowsky, S.A., van Dongen, J.L.J., Hackeng, T.M., Merkx, M., Meijer, E.W.  Multivalent peptide and protein dendrimers using native chemical ligation
 Angew. Chem. Int. Ed. (2005) 44, 5052-5057 

16. de Groot, M.T., Merkx, M., Wonders, A.H., Koper, M.T.M.
Electrochemical reduction of NO by hemin adsorbed at pyrolitic graphite
J. Am. Chem. Soc. (2005) 127, 7579-7586

15. Evers,T.H., Merkx, M.
Successful recombinant production of Allochromatium vinosum cytochrome c' requires coexpression of cmm genes in heme-rich Escherichia coli
Biochem. Biophys. Res. Commun. (2005) 327, 668-674 

2004

14. Sazinsky, M.H., Merkx, M., Cadieux, E., Tang, S.Y., Lippard, S.J.
Preparation and X-ray structures of metal-free, dicobalt and dimanganese forms of soluble methane monooxygenase hydroxylase from Methylococcus capsulatus (Bath)
 Biochemistry (2004) 43, 16263-16276
 
13. van Dongen, E.M.W.M, Klomp, L.W.J., Merkx, M.
Copper-dependent protein-protein interactions studied by yeast two-hybrid analysis
Biochem. Biophys. Res. Commun. (2004) 323, 789-795
 
12. Rudd, D.J., Sazinsky, M.H., Merkx, M., Lippard, S.J., Hedman, B., Hodgson, K.O.
Determination by X-ray absorption spectroscopy of the Fe-Fe separation in the oxidized form of the hydroxylase of methane monooxygenase alone and in the presence of MMOD
Inorg. Chem. (2004) 43, 4579-4589

Before 2004

11. Merkx, M., Lippard, S.J.
Why OrfY? Characterization of MMOD, a long overlooked component of the soluble methane monooxygenase from Methylococcus capsulatus (Bath)
 J. Biol. Chem. (2002) 277. 5858-5865    

10. Merkx, M., Kopp, D.A., Sazinsky, M.H., Blazyk, .JL., Müller, J., Lippard, S.J.
Dioxygen activation and methane hydroxylation by soluble methane monooxygenase: A tale of two irons and three proteins
 Angew. Chem. Int. Ed. (2001) 40, 2782-2807     
 
9. Stahl, S.S., Francisco, W.A., Merkx, M., Klinman, J.P., Lippard, S.J.
Oxygen kinetic isotope effects in soluble methane monooxygenase
J. Biol. Chem. (2001) 276, 4549-4553
 
8. Merkx, M. & Averill, B.A.
Probing the role of the trivalent metal in phosphatases containing AlIIIZnII and InIIIZnII active sites, including the first example of an active aluminum enzyme
J. Am. Chem. Soc. (1999) 121, 6683-6689     
 
7. Merkx, M., Pinkse, M.W.H. & Averill, B.A.
Evidence for nonbridged coordination of p-nitrophenyl phosphate to the dinuclear Fe(III)-Fe(II) center in bovine spleen purple acid phosphatase during enzymatic turnover
Biochemistry (1999) 38, 9914-9925      

6. Pinkse, M.W.H., Merkx, M. & Averill, B.A.
Fluoride inhibition of bovine spleen purple acid phosphatase: characterization of a ternary enzyme-phosphate-fluoride complex as model for the active enzyme-substrate-hydroxide complex
Biochemistry (1999) 38, 9926-9936          

5. Merkx, M. & Averill, B.A.
Ga3+ as a functional substitute of Fe3+: preparation and characterization of the Ga3+Fe2+- and Ga3+Zn2+-forms of bovine spleen purple acid phosphatise
Biochemistry (1998) 37, 8490-8497      
 
4. Merkx, M. & Averill, B.A.  
The activity of oxidized bovine spleen purple acid phosphatase is due to an Fe(III)Zn(II)-’impurity’
Biochemistry (1998) 37, 11223-11231
 
3. Kooter, I.M., Pierik, A.J., Merkx, M., Averill, B.A., Moguilevsky, N., Bollen, A. & Wever, R.
Difference fourier transform infrared evidence for ester bonds linking the heme group in myeloperoxidase, lactoperoxidase, and eosinophil peroxidase”
J. Am. Chem. Soc. (1997) 119, 11542-11543

2. de Lange, F., Merkx, M., Bovee-geurts, P.H.M., Pistorius, A. & de Grip, W.J.
Modulation of the metarhodopsin I/ metarhodopsin II equilibrium of bovine rhodopsin by ionic strength. Evidence for a surface-charge effect
Eur. J. Biochem. (1997) 243, 174-180        
 
1. Janssen, J.J.M., Bovee-geurts, P.H.M., Merkx, M. & de Grip, W.J.
Histidine tagging both allows convenient single-step purification of bovine rhodopsin and exerts ionic strength-dependent effects on its photochemistry
J. Biol. Chem. (1995)  270, 11222-11229