MSc. Thesis presentation Rob Driessen

Date:
17 December
Time:
10:00 - 10:30
Location:
Gem.-Z. 4.24

Rob Driessen wil present his MSc. Thesis work entitled 'Gas plasma modification for in situ tissue engineering'

Abstract

Heart valve in situ tissue engineering focusses on implanting smart biodegradable scaffolds that recruit host cells and stimulates subsequently neotissue formation as the scaffold degrades. Polycaprolactone (PCL) is a widely used hydrophobic scaffold material that requires functionalized prior to implantation. Gas plasma modification of the scaffold is fast, cost efficient, superficial and can be used as a sterilization method. In this study, PCL scaffold material is plasma modified with different plasmas aiming to improve adipose derived stem cell (ADSC) adhesion. Here it is shown that ADSC adhesion is most influenced by surface chemistry and not by surface energy. In contrast to what is found in literature stainings for actin and vinculin and a wash assay show no difference in cell adhesion for increasing surface energies. Plasma polymerization with RGD mimicking groups show minor changes in surface energy and improves cell adhesion significantly. ADSCs on this plasma polymerized substrate spread more and cover more surface area. Both actin/vinculin and zyxin/α‐actinin stainings confirm these findings with stainings after 1, 2 and 6 hours showing even more distinct differences in cell adhesion with cells on PCL control substrates. This study shows that ADSC adhesion to PCL scaffolds for in situ tissue engineering can significantly be improved if it is plasma polymerized with an RGD mimicking monomer is introduced into the plasma.