8MM10 - Microscopy for Biological Samples
The course is meant for students that may have to do light microscopy on biological samples for their internship(s) or MSc-project, and for students that are interested in biomedical applications of optics in general. After completion of this course one has obtained a thorough background in the possibilities and limitations of various optical-microscopic techniques, in particular the possibilities and limitations related to biological samples.
The student knows:
- the most important physical aspects of light microscopy
- the relative importance of the various factors
- the most important principles of special microscopic techniques as polarization microscopy, phase contrast and differential interference contrast
- the most important physical aspects of fluorescence
- the most important physical aspects of fluorescence microscopy: epi-fluorescence, confocal laser scanning microscopy and multi-photon laser scanning microscopy
- the possibilities of specific techniques such as second harmonic generation and fluorescence techniques such as fluorescence lifetime imaging, stochastical techniques and structured illumination (fluorescence) techniques.
- the possibilities and limitations of all these microscopy techniques
- the relationship between microscopic imaging and electronically acquired images
- the possibilities and dangers of application of these techniques to living tissue
The student can:
- work with a standard microscope
- describe and select in a correct way the most important properties of the various microscope types
- advise of the choice of various microscope parts on the basis of product information
- advise of the coherence of the various components of a microscope
- indicate what type of microscope is required for a certain research question
- understand and interpret literature in the field of microscopy
- advise of the use of various types of microscopes for biological samples.
Responsible lecturer: M.C. (Mark) van Turnhout.
Co-lecturer: L. (Lorenzo) Albertazzi.