Bone is a dynamic tissue that is able to adapt to its environment by a process called bone remodeling. With ageing, this adaptive response is reduced, creating an imbalance between bone formation and resorption. This abnormal bone remodeling is a primary cause of bone diseases, such as osteoporosis and Paget’s disease. New treatments for these bone diseases are developed by testing their effects in vitro with 2D experiments, followed by in vivo animal studies. The outcomes of these 2D experiments are often not representative of what is observed in vivo, because the complexity of the natural bone environment, such as the 3D structure, vascularization and the variety of cell types and their interactions, is missing. The culture of bone explant material addresses this problem because it maintains the native 3D environment. This so-called ex vivo bone culture provides a powerful platform to study fundamental processes, such as bone remodeling, in healthy and diseased bone. With this ex vivo bone model, processes on different levels can be analyzed, including interactions between cells as well as effects of mechanical loading or drugs on mineralization. In this project, an ex vivo bone platform will be established that could potentially contribute to a better understanding of bone remodeling and might eventually be used for screening and optimization of new therapies.